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Will the South China tiger make its way back to the wild again?

Photo credit: Yifu Liu.

In the provinces of South China, there once roamed a unique subspecies of tiger. The South China tiger (Panthera tigris amoyensis) is ecologically, morphologically, and genetically different from other tigers.

But, unfortunately, you will no longer find one of these tigers in the wild. All that remain are in captivity. The South China tiger is listed as “Critically Endangered (Possibly Extinct in the Wild)” in the IUCN Red List of Threatened Species.

Photo credit: Ge Sun.

Habitat loss and human-tiger conflict have led to the collapse of the wild population. They haven’t been seen in the wild in over three decades.

The South China tiger is considered functionally extinct. This means there aren’t enough tigers left in the wild for a sustainable population, making the captive population very valuable. It is important to identify and maintain the South China tiger in captivity if, someday, South China tigers are to be reintroduced into the wild.

Zhang, Xu, and colleagues (2019) from the Chengdu Research Base of Giant Panda Breeding, Peking University, and nearly all zoos in China maintaining the last surviving South China tigers, looked at the largest sample of captive tigers to date. They looked at both mitochondrial DNA (4687-bp) and microsatellites (30) of 87 captive South China tigers from 14 zoos or breeding centers. That is 75% of the total population!

Their goal was to determine if there is potential for using the captive population to reintroduce the South China tiger to the wild.

Genetic ancestry is a way of looking at an individual’s past by how they share genes with others in the population. To identify the ancestry of the captive South China tiger, Zhang et al. applied a system that uses genetics of captive tigers worldwide, called verified subspecies ancestry (VSA; Luo et al. 2008, 2010a, 2010b).

The Chinese Association of Zoological Gardens has been keeping track of all the South China tigers in captivity in a studbook. Studbooks are used by zoos to follow family histories over generations.

Like 23andMe for tigers, each tiger’s genetic ancestry was found and then matched with its family history from the studbook to make a pedigree that shows all the relationships within the captive population (Figure 1) and how these captive tigers are related to other subspecies.

Figure 1. Pedigree of the 2 major lines forming the captive South China tiger population in China and genetic ancestry of each individual based on mtDNA haplotype and microsatellite composite genotypes from 30 loci. (Zhang et al. 2019)

The South China tiger is the most closely related to the Indochinese tiger. Zhang et al. found three VSA mitochondrial haplotypes (the South China tiger, Indochinese tiger, and Amur tiger). Tigers with the South China and Indochinese haplotypes could be traced to two individual wild-caught females. For the South China tigers, this founding female was #7 from Guizhou. The Indochinese tigers, #26 from Fujian.

From 1955 to 1970, South China tigers from Fujian and Guizhou were brought into the Chongqing and Suzhou Zoos. Now, the 144 living South China tigers are descendants of six wild founders (two males and four females) from those two zoos. These two lines of tigers were maintained until 1995 when the zoos started breeding them to increase genetic diversity and decrease inbreeding depression.

Seventy-four of the sampled tigers fell into these two lines. The remaining 18 tigers descended from the Suzhou line but had various degrees of admixture, or genetic evidence of other subspecies.

This “foreign” DNA from Bengal, Amur, and Indochinese tigers was unintentionally introduced into the Suzhou line when tigers with unknown origins were introduced into the captive South China tiger population, evidence of possible mismanagement.

However, taking those 18 tigers out of the population, the remaining South China tigers show levels of genetic variability like species with proven successful captive conservation programs (Luo et al. 2008).

The captive South China tiger population has the potential for population growth and, with well-coordinated genetic management, eventual reintroduction to the wild.

Principal investigator Dr. Shu-Jin Luo says that “this study has provided a full picture of the genetic status of the captive South China tiger population and sheds new light on the possible candidates to use, should reintroduction into their original range come into reality.”

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Caitlin Curry is a Postdoctoral Scientist in the Conservation Genetics Lab at Omaha’s Henry Doorly Zoo & Aquarium doing research on lemur biodiversity in Madagascar. She studied conservation and population genetics in the Interdisciplinary Program of Genetics at Texas A&M University where her dissertation research focused on biodiversity of the African lion over time using ancient DNA from historical lions found in museum collections to explore how genetic diversity has changed in lions over the past century. You can learn more about her work on her website.

 

References:

Zhang W et al. 2019. Sorting Out the Genetic Background of the Last Surviving South China Tigers. Journal of Heredity. 110(6): 641-650.

Luo SJ et al. 2008. Subspecies genetic assignments of worldwide captive tigers increase conservation value of captive populations. Current Biology. 18:592-596.

Luo SJ et al. 2010a. Applying molecular genetic tools to tiger conservation. Integrative Zoology. 5:351-362.

Luo SJ et al. 2010b. What is a tiger? Genetics and phylogeography. In: Tilson RL, Nyhus PJ, editors. Tigers of the world, the science politics and conservation of Pathera tigris. 2nd ed. New York: Elsevier/Academic Press. p. 35-51.

 

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